Tamara Lynn Hendrickson (dy8142)

Chemistry

Chem 365

Indirect tRNA aminoacylation, accuracy mechanisms in protein biosynthesis, membrane proteins, protein-protein interactions, enzyme kinetics, diversity in STEM, women in STEM

Our research group uses a multidisciplinary approach to investigate indirect tRNA aminoacylation pathways in pathogenic bacteria. We draw on techniques from biochemistry, biophysical chemistry, and molecular and microbiology to understand these complex macromolecular processes.

Indirect tRNA aminoacylation

There are twenty proteinaceous amino acids that are commonly used by all organisms. In most cases, including humans, yeast, and E. coli, there are twenty aminoacyl-tRNA synthetases, with one enzyme responsible for attaching each encoded amino acid to the correct tRNA(s). However, in many organisms glutaminyl-tRNA synthetase and asparaginyl-tRNA synthetase (GlnRS and AsnRS, respectively) are missing. In these cases, the corresponding aminoacyl-tRNAs, Gln-tRNAGln and Asn-tRNAAsn, are made indirectly via transamidation of Glu-tRNAGln and Asp-tRNAAsn. This biosynthetic pathway requires the presence of two misacylating AARSs and a glutamine-dependent amidotransferase (Adt).

We are investigating the indirect biosynthesis of Gln-tRNAGln and Asn-tRNAAsn in  pathogenic bacteria, including H. pylori and S. aureus We are interested in understanding the evolution of direct versus indirect tRNA aminoacylation pathways as well as the mechanisms that are used by H. pylori to prevent misacylated tRNAs from entering the ribosome prior to conversion to their accurately aminoacylated counterpoints.

2018-2019 Drexel ELATE Fellow

2015-2016 WSU College of Liberal Arts and Sciences Excellence in Teaching Award

2011 Chair and Host, 2011 International Conference on Aminoacyl-tRNA Synthetases

2009-2010 WSU Career Development Chair Award

2007 American Cancer Society Research Scholar Award

2000 Research Corporation Innovation Award

Ness, Travis J.; Gamage, D.G.; Ekayanaka, S.A.; Hendrickson, T.L. "A soluble, minimalistic glycosylphosphatidylinositol transamidase (GPI-T) retains transamidation activity." 2022, Biochemistry, 61, 1273-1285. DOI: 10.1021/acs.biochem.2c00196.

Humphreys, I. R.; Pei, J.; Baek, M.; Krishnakumar, A.; Anishchenko, I.; Ovchinnikov, S.; Zhang, J.; Ness, T. J.; Banjade, S.; Bagde, S. R.; Stancheva, V. G.; Li, X.-H.; Liu, K.; Zheng, Z.; Barrero, D. J.; Roy, U.; Kuper, J.; Fernández, I. S.; Szakal, B.; Branzei, D.; Rizo, J.; Kisker, C.; Greene, E. C.; Biggins, S.; Keeney, S. Miller, E. A.; Fromme, C. J.; Hendrickson, T. L.; Cong, Q.; Baker, D. “Computed structures of core eukaryotic protein complexes.” 2021, Science, 10, eabm4805. DOI: 10.1126/science.abm4805.

Li, Y.-Y.; Cai, R.-J.; Yang, J.-Y.; Hendrickson, T. L.; Xiang, Y.; Javid, B. “Clinically relevant mutations of mycobacterial GatCAB inform regulation of translational fidelity.” 2021, mBIO, 12, e0110021. DOI: 10.1128/mBio.01100-21.

Hendrickson, T. L.; Wood, W. N.; Rathnayake, U. “Did amino acid side chain reactivity dictate the timing of aminoacyl-tRNA synthetase and protein code evolution?” 2021, 12, 409. DOI: 10.3390/genes12030409 (https://www.mdpi.com/2073-4425/12/3/409).

Rathnayake, U. M.; Hendrickson, T. L. “Bacterial aspartyl-tRNA synthetases have moderate glutamyl-tRNA synthetase activity.” 2019, Genes, 10, E262. doi: 10.3390/genes10040262.

Gamage, D. G.; Varma, Y.; Meitzler, J. L.; Morissette, R.; Ness, T. J., Hendrickson, T. L. “The soluble domains of Gpi8 and Gaa1, two subunits of glycosylphosphatidylinositol transamidase (GPI-T), assemble into a complex.” 2017, Arch. Biochem. Biophys. 633, 58-67.
Hendrickson, T. L. "Old enzymes versus new herbicides." 2018, J. Biol. Chem., 293, 7892-7893.

Zhao, L., Rathnayake, U. M., Dewage, S. W., Wood, W. N., Veltri, A. J., Cisneros, G. A., Hendrickson, T. L. "Characterization of tunnel mutants reveals a catalytic step in ammonia delivery by an aminoacyl-tRNA amidotransferase." 2016, Febs Lett. 590, 3122-3132.

Hendrickson, T. L. "Integrating responsible conduct of research education into undergraduate biochemistry and molecular biology laboratory curricula." 2015, Biochem. Mol. Biol. Educ., 43, 68-75.

Gamage, D. G., Hendrickson, T. L. "GPI transamidase and GPI anchored proteins: oncogenes and biomarkers for cancer." 2013, Crit. Rev. Biochem. Mol. Biol., 48, 446-464.

Silva, G. N., Fatma, S., Floyd, A. M., Fischer, F., Chuawong, P., Cruz, A. N., Simari, R. M., Joshi, N., Kern, D., Hendrickson, T. L. "A tRNA-independent mechanism for transamidsome assembly promotes aminoacyl-tRNA transamidation." 2013, J. Biol. Chem., 288, 3816-3822.